1998 - Ph.D. Microbiology and Molecular Genetics, The Medical College of Wisconsin
Core Facility Overview
Dr. Tvinnereim oversees the operation and management of designated core facility equipment and BSL3 laboratory in Biomedical Research. The core facility of UT Health Northeast supports the innovative research done at UT Health by providing specialized equipment as well as expertise and training required to optimize the data obtained from this equipment.
Core Facility Equipment
Flow Cytometers: The flow cytometry core facility is equipped with three flow cytometers. A Becton Dickinson FACSCalibur in the Biosafety Level 3 labs has 3 lasers (488 nm and 633 nm) and is able to analyze 4 colors in cells potentially infected with biosafety level 3 pathogens. A similarly equipped second Becton Dickinson FACSCalibur is available for researchers to run their own samples or to have samples run by the core facility. A Becton Dickinson FACSAria I with 2 lasers (488 and 633) that is able to analyze and sort cells stained with up to 7 colors.
The flow cytometry core allows users to analyze thousands of cells per second using fluorescent molecules. Common applications of flow cytometry include analysis or sorting experiments studying: cell phenotype, cell viability including apoptosis measurements, DNA cell cycle analysis, and transfection efficiency
Micro CT Scanner: The GE Locus eXplore micro CT scanner allows CT imaging of mice at resolutions as high as 27 microns. The micro CT scanner allows measurement of the amount of inflammation in the lungs during respirator infections such as influenza and pneumonia. In addition, analysis of bone structure can be performed using the GE Locus eXplore.
Flexivent: The Flexivent is a ventilator for mice that allows measurements of pulmonary function to be performed. The Flexivent allows researchers to determine whether treatments for lung injury or infection have resulted in improved lung function such as how easy is it for the lungs to expand and whether the lungs are constricted.
Madison Aerosol Exposure Chamber: The Madison Aerosol Exposure Chamber allows investigators to infect mice with Mycobacterium tuberculosis. The Madison Chamber puts the bacteria into small air borne droplets of liquid which allows the mice to become infected by breathing in the bacteria. This is the same route of infection that occurs in humans.
2014 Krupa A, Fol M, Rahman M, Stokes KY, Florence JM, Leskov IL, Khoretonenko MV, Matthay MA, Liu KD, Calfee CS, Tvinnereim A, Rosenfield GR, Kurdowska AK., Silencing Bruton’s tyrosine kinase in alveolar neutrophils protects mice from LPS/immune complex induced acute lung injury., Am J Physiol Lung Mol physiol. 2014 Sept 15; 207)6):L435-48..
2014 Dhiman R, Venkatasubramanian S., Paidipally P., Barnes P.F., Tvinnereim A., Vankayalapati R., Interleukin 22 Inhibits Intracellular Growth of Mycobacterium tuberculosis by Enhancing Calgranulin A Expression., J Infect Dis. 2013 Oct 4
2012 Karandashova S., Florova G., Azghani A.O., Komissarov A.A., Koenig K., Tucker T.A., Allen T.C., Stewart K., Tvinnereim A., Idell S., Intrapleural Adenoviral Deliverary of Human PAI-1 Exacerbates TCN-Induced Pleural Injury in Rabbits. Am. J. Respir. Cell. Mol. Biol. 2012 Sep 20
2012 Dhiman R., Periasamy S., Barnes P.F., Jaiswal A.G., Paidipally P., Barnes A.B., Tvinnereim A., Vankayalapati R., NK1.1+ cells and IL-22 regulate vaccine-induced protective immunity against challenge with Mycobacterium tuberculosis., J. Immunnol. 2012 Jul 15;189(2):897-905.
2011 Sever-Chroneos Z., Tvinnereim A., Hunter R.L., Chroneos Z.C., Prolonged survival of scavenger receptor class A-deficient mice from pulmonary Mycobacterium tuberculosis infection. Tuberculosis. 2011 Dec; 91 Suppl 1: S69-74.
2011 Periasamy, S., Dhiman, R., Barnes, P.F., Paidipally, P., Tvinnereim, A., Bandaru, A., Valluri, V.L., Vankayalapati, R. Programmed Death-1 (PD-1) and cytokine inducible SH2-containing protein (CISH) dependent expansion of regulatory T cells upon stimulation with Mycobacterium tuberculosis. In Press
2011 Dhiman, R., Bandaru, A., Barnes, P.F., Sah, S., Tvinnereim, A., Nayak, R. C., Paidipally, P., Valluri, V., Rao, V.M., Vankayalapati, R. c-Maf-dependent growth of Mycobacterium tuberculosis in a CD14hiCD16- subpopulation of monocyte-derived macrophages. J. Infect. Dis. 2011 May1;203(9):125601263
2011 Feng, Y., Kong, Y., Barnes, P.F., Huang, F.F., Klucar, P., Wang, X., Samten, B., Sengupta, M., Machona, B., Donis, R., Tvinnereim, A.R., and Shams, H. Exposure to Cigarette Smoke Inhibits the Pulmonary T cell Response to Influenza and Mycobacterium tuberculosis. Infect. Immun. 2011 Jan;79(1):229-37
2010 Guo, J., Rahman, M., Cheng, L., Zhang, S., Tvinnereim, A., and Su, D.M. Morphogenesis and maintenance of the 3D thymic medulla and prevention of nude skin phenotype require FoxN1 in pre- and post-natal K14 epithelium. J. Mol. Med. 2010 Nov 26.
2009 Klucar P, Barnes PF, Kong Y, Howard ST, Pang X, Huang FF, Tvinnereim AR, Samten B, Shams H. Vaccination strategies to enhance local immunity and protection against Mycobacteriun tuberculosis. Vaccine. 27(12):1816-1824.